Xenbase Image ID: 175544

Figure 6: The expression of first and second heart field markers is affected by Kmt2d loss of function. Embryos were injected with 2.5 ng MO (Kmt2d or 5 bp mismatch control) in combination with 100 pg LacZ RNA in one dorsal blastomere at the 4-cell stage and analyzed by Tbx20 or Isl1 in situ hybridization. A Schematic drawing of Tbx20 expression in the first heart field at stage 29. B-E Embryos analyzed by Tbx20 in situ hybridization shown from the ventral side; asterisks indicate the injected side. B Uninjected control embryo. C Embryo injected with mismatch control MO. D,E Kmt2d morphant embryos showing a reduction as well as a posterior shift of Tbx20 expressing cells on the injected side. F Graph summarizing three independent injection experiments, standard errors of the means and numbers of analyzed embryos are indicated for each column. *** P-value in a Student’s t-test < 0.001. G Schematic drawing of Isl1 expression in the second heart field at stage 29. H-K Embryos analyzed by Isl1 in situ hybridization. H Uninjected control. I Mismatch control. J,K Kmt2d morphants with reduced expression of Isl1. L Graph summarizing three independent experiments, s.e.m as well as numbers of analyzed embryos are indicated. ** P-value in a Student’s t-test < 0.01. Image published in: Schwenty-Lara J et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue tbx20.L XTBX20 X. laevis Sometime during NF stage 1 to NF stage 29 and 30 primary heart field isl1.L islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 29 and 30 secondary heart field Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + kmt2d MO + NF29/30 (in situ hybridization) Fig. 6 D E F Anatomical Phenotype abnormal development of primary heart field Disease congenital heart disease Kabuki syndrome Expression Phenotype decreased amount tbx20.L expression in primary heart field Xla Wt + kmt2d MO + NF29/30 (in situ hybridization) Fig. 6 J K L Anatomical Phenotype abnormal development of secondary heart field Disease congenital heart disease Kabuki syndrome Expression Phenotype decreased amount isl1.L expression in secondary heart field Larger Image Printer Friendly View

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