Xenbase Image ID: 175545

Figure 7: Nkx2.5 expression is downregulated in Kmt2d morphant embryos. Embryos were injected with 2.5 ng MO Kmt2d (or 2.5 ng 5 bp mismatch control) or 5 ng splice MO (or 5 ng control MO) in combination with 100 pg LacZ RNA in one dorsal blastomere at the 4-cell stage. Whole mount in situ hybridization of st. 28 embryos was performed for the cardiac lineage marker Nkx2.5. Asterisks mark the injected side; all embryos are shown from the ventral side. A,B,C Uninjected control and embryos injected with 2.5 ng mismatch MO (B) or 5 ng control MO (C) showing normal expression of Nkx2.5 on both sides. D,E Kmt2d MO (D) and splice MO (E) injected embryos displaying a strongly reduced expression of Nkx2.5 (arrow; dashed line marks ventral midline). F Schematic drawing of Nkx2.5 expression in a st. 28 embryo. G Graph summarizing three independent experiments with the number of analyzed embryos and s.e.m. indicated for each condition. ** P-value in a Student’s ttest < 0.01; * P-value in a Student’s t-test < 0.05. Image published in: Schwenty-Lara J et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue nkx2-5.L AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Throughout NF stage 28 heart primordium Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + kmt2d MO + NF28 (in situ hybridization) Fig. 7 D G Anatomical Phenotype abnormal development of heart primordium Disease congenital heart disease Kabuki syndrome Expression Phenotype decreased amount nkx2-5.L expression in heart primordium Xla Wt + kmt2d MO + NF28 (in situ hybridization) Fig. 7 E G Anatomical Phenotype abnormal development of heart primordium Disease congenital heart disease Kabuki syndrome Expression Phenotype decreased amount nkx2-5.L expression in heart primordium Larger Image Printer Friendly View

Return to previous page