Xenbase Image ID: 175546

Figure 8: The development of Isl1-positive cardiac progenitor cells is largely unaffected in Kmt2d morphants. Embryos were injected with 2.5 ng MO Kmt2d (or 2.5 ng 5 bp mismatch control) or 5 ng splice MO (or 5 ng control MO) in combination with 100 pg LacZ RNA in one dorsal blastomere at the 4-cell stage. At early neurula stage 14/15 embryos were analyzed for Isl1 expression by in situ hybridizationA Uninjected control embryo; left (anterior view), right (dorsal view) of the same embryo. B Mismatch MO injected embryo. C Kmt2d MO injected embryo. D Graph summarizing three independent experiments. Standard errors of the means and numbers of analyzed embryos are shown for each condition; n.s. not significant in a Student’s t-test. E Uninjected control embryo. F Control MO injected embryo. G Splice MO injected embryo. H Graph summarizing three independent experiments. Standard errors of the means and numbers of analyzed embryos are shown for each column; n.s. not significant in a Student’s t-test. Image published in: Schwenty-Lara J et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue isl1.L islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 14 cardiac mesoderm Image source: Published Larger Image Printer Friendly View

Return to previous page