Xenbase Image ID: 175560

Fig. 6. A-B′) Representative control and DspMO1 morphant trunk epidermis labeled with keratin (A,B; green), beta-catenin (A′,B’; pink) and merge (A′,B′). Scale bars = 25 μm. Beta-catenin labeling (pink) was used to provide a marker of the cortical region of the cell. C,D) alpha-tubulin labeling of cells from control (C) and DspMO1 morphants (D). Scale bars = 25 μm. E,F) Phalloidin labeling of F-actin from control (C) and DspMo1 morphants (D). Scale bars = 25 μm. G) Schematic of the experimental design for H-I′. H-H′) Representative control trunk epidermis labeled with E-cadherin (J), showing the fluorescein labeled MO (H′) and merge (H′). Scale bar = 25 μm. K-K′) Representative control trunk epidermis labeled with E-cadherin (I), showing the Fluorescein labeled MO (I′) and merge (I′). Scale bar = 25 μm. Abbreviations = Ecad = E-cadherin. Image published in: Bharathan NK and Dickinson AJG (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue krt61.L krt5, krt5.2 X. laevis Sometime during NF stage 29 and 30 to NF stage 31 epidermis ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 29 and 30 to NF stage 31 epidermis tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Sometime during NF stage 19 to NF stage 20 epidermis cdh1.L arc-1, cd324, cdhe, e-cadherin, ecad, lcam, uvo, uvomorulin, XTCAD-1 X. laevis Throughout NF stage 22 epidermis Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dsp MO + NF29/30-31 (immunohistochemistry) Fig. 6 B B' B" Anatomical Phenotype abnormal epidermis morphology Disease skin disease Expression Phenotype mislocalised krt61L expression in epidermis Xla Wt + dsp MO + NF19-20 (immunohistochemistry) Fig. 6 D Anatomical Phenotype abnormal epidermis morphology abnormally localised microtubule Disease skin disease Expression Phenotype mislocalised tuba4b.L expression in epidermis Xla Wt + dsp MO + NF22 (immunohistochemistry) Fig. 6 I I' I'' Anatomical Phenotype abnormal epidermis morphology Disease skin disease Expression Phenotype decreased amount cdh1.L expression in epidermis Larger Image Printer Friendly View

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