Xenbase Image ID: 175561

Fig. 7. DP-NTP expression in X. laevis embryos. A) Schematic of the major domains of desmoplakin in wildtype (WT) and DP-NTP construct. B) Desmoplakin labeled with an antibody B′) DP-NTP-GFP expression B′) Merge of B and B’. C,D) Lateral views of representative control (C) and DP-NTP expressing embryos. E-E′) Trunk epidermis of a representative control embryo, labeled with tubulin (pink, E) and phalloidin (green) merged with tubulin (E′). F-F′) Trunk epidermis of a representative DP-NTP expressing embryo, labeled with tubulin (pink, F) and phalloidin (green) merged with tubulin (F′). G,H). Keratin localization in the trunk epidermis of a representative control (G) DP-NTP expressing embryo (H). Scale bars = 25 μm. Image published in: Bharathan NK and Dickinson AJG (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Sometime during NF stage 24 to NF stage 29 and 30 multiciliated epidermal cell cilium krt61.L krt5, krt5.2 X. laevis Sometime during NF stage 24 to NF stage 29 and 30 epidermis Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Hsa.DSPdel + NF28-29/30 (whole-mount microscopy) Fig. 7 D Anatomical Phenotype decreased size of the embryo increased pigmentation in the epidermis Disease skin disease Xla Wt + Hsa.DSPdel + NF26-28 (immunohistochemistry) Fig. 7 F F' Anatomical Phenotype abnormal development of multiciliated cell abnormally decreased number of multiciliated epidermal cell abnormally localised multiciliated cell Disease skin disease Xla Wt + Hsa.DSPdel + NF29/30-31 (immunohistochemistry) Fig. 7 H Disease skin disease Expression Phenotype decreased amount krt61L expression in cortical actin cytoskeleton Larger Image Printer Friendly View

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