Xenbase Image ID: 175564

Supplemental Figure 2: Characterization of Dsp expression. A) RT-PCR of Dsp at embryonic stages as well positive controls in the adult skin and heart. RT+ = reverse transcriptase was added, RT- = reverse transcriptase was not added. Primer sequences available upon request. B-E''') Immunofluorescence of Dsp (green) in the epidermis from various embryonic stages. Dapi (blue) and beta-catenin (pink) labeling were used as a counter stain. F-F''') A 3D rendering of images as shown in E-E''' were created and then rotated 90 degrees. G-G''') st. 30 where only the inner epidermal layer of cells are shown. All scale bars = 40µm Image published in: Bharathan NK and Dickinson AJG (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue dsp.L X. laevis Sometime during NF stage 10 to NF stage 11 ectoderm dsp.L X. laevis Sometime during NF stage 19 to NF stage 20 epidermis dsp.L X. laevis Sometime during NF stage 24 to NF stage 25 epidermis dsp.L X. laevis Throughout NF stage 32 epidermis outer layer ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 10 to NF stage 11 ectoderm ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 19 to NF stage 20 epidermis ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Sometime during NF stage 24 to NF stage 25 epidermis ctnnb1.L B-catenin, beta catenin, beta-catenin, ctnnb1-a, ctnnb1-b, β-catenin X. laevis Throughout NF stage 32 epidermis outer layer epidermis inner layer Image source: Published Larger Image Printer Friendly View

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