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Supplemental Figure 4: DspMO1 targeted to the face, eye and fin causes developmental defects. All scale bars= 85µm. A) Schematic showing the blastomere injected with MO to target the face. B-B’’) Frontal view of the face of a representative embryo with control MO in the face. Shows an embryo with light microscope (B), fluorescent (B’) and merge (B’’). The embryonic mouth forms normally. C-C’’) Frontal view of the face of a representative embryo with DspMO1 in the face. Shows embryo with light microscope (C), fluorescent (C’) and merge (C’’). The embryonic mouth does not form (arrow). D) Schematic showing the blastomere injected with MO to target the eye. E-E’’) Lateral view of the head of a representative embryo with control MO in the eye. Shows an embryo with light microscope (E), fluorescent (E’) and merge (E’’). F-F’’) Lateral view of the head of a representative embryo with DspMO1 in the eye, shows embryo with light microscope (F), fluorescent (F') and merge (F''). The abnormal eye is indicated by an arrow. G) Schematic showing the blastomere injected with MO to target the dorsal fin. H-H'') Lateral view of the head of a representative embryo with control MO in the dorsal fin. Shows an embryo with light microscope (H), fluorescent (H') and merge (H''). I-I'') Lateral view of the head of a representative embryo with DspMO1 in the fin, shows embryo with light microscope (I), fluorescent (I') and merge (I''). The abnormal fin is indicated by an arrow. Image published in: Bharathan NK and Dickinson AJG (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
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