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Figure 1—figure supplement 1 Quantification of unspliced vs. spliced transcripts in MO-injected embryos. (a) Schematic representation of the dkk2 locus. The position of the splice blocking MO (Dkk2SMO) is shown (red line). The PCR primers used to analyze the spliced (E1/E2) and unspliced (I2/I3) transcripts are indicated. (b) qRT-PCR analysis of fold increase in unspliced (intron one retention) vs. spliced transcripts upon injection of increasing doses of MO. (c) Increasing amount of MO resulted in a dose-dependent decrease in snai2 and sox10 expression in these embryos. A representative experiment is shown. Image published in: Devotta A et al. (2018) © 2018, Devotta et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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