Xenbase Image ID: 175666

Figure 1 Severing of the TGN and its effects on lens regeneration. A: Normal innervation of the larval Xenopus cornea (st 52) labeled with anti‐acetylated alpha‐tubulin antibody (green fluorescence). B: Schematic diagram of Xenopus cranial innervation on the left side of the head. The TGN branches from the brain and travels through the head region, where some branches serve the cornea (red branches; see Perry et al., 2013 for full description). The site containing the main nerve trunk that was severed for nerve cut experiments is noted with scissors. C: Time line of the TGN cutting experiments in conjunction with the lentectomy procedure to remove the lens. Time (days) is represented along the horizontal axis. D: Xenopus cornea labeled with anti‐acetylated alpha‐tubulin antibody (green fluorescence) at the end of the TGN cutting/lens regeneration experiment outlined in C. Note reduced labeling of main nerve branches and significantly reduced nerve structures in the central cornea region, as compared to A. Some fibers from both the mandibular nerve and the recurrent branch of the nasociliary nerve are seen. E: Representative transverse section from eye presented in D. The regenerated lens is labeled with anti‐lens antibody (labeled with ln; red fluorescence). F: A second case showing an anti‐acetylated alpha‐tubulin‐labeled (green fluorescence) cornea at the end of the TGN cutting/lens regeneration experiment outlined in C. Note little if any innervation from the mandibular nerve on the posterior side (left), but some innervation from the nasociliary nerve is present on the anterior side (right). G: Representative transverse section from eye presented in E, where the regenerated lens has been labeled with anti‐lens antibody (labeled with ln; red fluorescence). H: Graphical representation of lens regeneration rates following TGN cuts compared to control animals without TGN cuts. Abbreviations: co, cornea; ion, infraorbital nerve; ln, lens; mn, mandibular nerve; mnb, mandibular nerve branch; on, optic nerve; rb, recurrent nerve branch; rt, retina; TGN, trigeminal nerve. P value = 0.2606. Error bars represent SD. Scale bar in A,D,F = 105 μm. Scale bar in E,G = 140 μm Image published in: Perry KJ et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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