XB-IMG-175683
Xenbase Image ID: 175683
Fig 1. X. laevis embryonic stages show VSP mRNA transcripts expression.(A-B) Semi-quantitative RT-PCR (sqRT-PCR) of a panel of X. laevis embryos (NF stage 12–40) using PCR primers specific for Xl-VSP1 (A) and Xl-VSP2 (B). Both Xl-VSP1 and Xl-VSP2 transcripts appear to accumulate by stage 36. No bands were seen without reverse transcriptase (bottom). All cDNAs were made with equal amounts of total RNA as determined by spectrophotometry and confirmed by agarose gel electrophoresis to visualize ribosomal RNA bands (S1 Fig). sqRT-PCR was repeated at least two times with at least two different embryonic cohorts. The expected PCR amplicon sizes are 389 bp for Xl-VSP1 and 478 bp for Xl-VSP2. Shown are representative gels. Image published in: Ratzan W et al. (2019) © 2019 Ratzan et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |