Xenbase Image ID: 175686

Fig 4. Xl-VSP protein is expressed on the lumenal surface of embryonic pronephroi.NF stage 42 Cdh17:GFP X. laevis embryos were sectioned and stained to test for VSP protein. Kidney tubules were identified by the presence of the Cdh17:GFP transgene (B, B'). Sections stained with anti-VSP (A) showed fluorescence on the lumenal surface (corresponding to the apical membrane) of the proximal kidney tubule cells (outlined in white). Anti-VSP staining was only observed in the presence of anti-VSP (A-F) and not in secondary antibody alone control sections (A'-F'). Panels (A, A') anti-VSP or no anti-VSP control; (B, B') GFP transgene; (C, C') fluorescence signal overlay of A, B and E; (D, D') bright field images; (E, E') Hoechst 33342 (to mark nuclei); (F, F') signal overlay of A, B, D and E. IHC was repeated three times with three different embryonic cohorts. Shown are representative sections. Scale bar = 25 μm. Image published in: Ratzan W et al. (2019) © 2019 Ratzan et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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