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Fig. 2. Neural Crest migration in control and sema3A-KD environments. a Procedure for grafting of NC cells. b Images at t0h and t6h of the time lapse movies corresponding to 4 h and 10 h after the graft, respectively. c Projections of all NC domains at t0h and t6h for all experimental conditions, note that overall distribution and dorso-ventral migration of control NC cells are affected in sema-KD background. d Graph plotting the distance migrated by NC cells in all conditions shown in b, c. 35 embryos (nCMO = 10, nSema3AMO = 11, nSema3A-gRNA-aCas9 = 7, nSema3A-gRNA-dCas9 = 7), from five independent experiments. ANOVA followed by multiple comparisons. ****p < 0.0001; ***p < 0.001. Scale bar 150 μ. Box and whiskers plot: the box extends from the 25th to the 75th percentile; the whiskers show the extent of the whole dataset. The median is plotted as a line inside the box. Source data are provided as a Source Data file Image published in: Bajanca F et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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