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Fig. 3. Sdf1 and Sema3A have antagonistic effects on cell dispersion. a Diagram explaining how NC explants were prepared. b Representative examples of explants at t0 (one hour after plating on Fibronectin) and +8 h. Note that cells exposed to Sema3A have a round morphology and tend to stay as small clusters, even when dispersion is rescued by Sdf1. c Distribution of explants areas per hour per experimental condition. A total of 229 explants (ncontrols = 50; nSdf1 = 32; nSema3A-15ng.mL = 29; nSema3A-15ng.mL+Sdf1 = 17; nSema3A-30ng.mL = 24; nSema3A-30ng.mL+Sdf1 = 14; nSema3A-60ng.mL = 47; and nSema3A-60ng.mL+Sdf1 = 16) from five independent experiments were used. Two-way ANOVA, matching: stacked, pairwise multiple comparisons. *p value < 0.05; **p value < 0.01; ***p value < 0.001; ****p value < 0.0001. Dotted line on the graphs represents the mean value for controls at 8 h, provided as a visual reference for comparison with other conditions. Scale bar on low magnification, 100 μ. Scale bar on zooms, 20 μ. Box and whiskers plot: the box extends from the 25th to the 75th percentile; the whiskers show the extent of the whole dataset. The median is plotted as a line inside the box. Source data are provided as a Source Data file Image published in: Bajanca F et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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