Xenbase Image ID: 175728

Figure 2. Conserved cysteines in Prdx1 are essential for pronephros development. (A) Three prdx1 mutants, namely, C53S (M1), C173S (M2), and C53S/C173S (M1/2), were subcloned using PCR-based site-directed mutagenesis. (B) Flag-tagged Prdx1* or HA-tagged prdx1 (200 pg) was co-injected into both blastomeres of two-cell stage embryos. Lysates from stage 12 embryos were used for immunoprecipitation. WT Prdx1 only showed in immune complex whereas mutant Prdx1 did not. (C) prdx1 MOs (40 ng) were co-injected with WT or mutant prdx1 (M1, M2 or M1/M2) into both blastomeres of two-cell stage embryos. Embryos at the stage 33 were used for whole-mount in situ hybridization, and proximal tubules in the pronephros were visualized with a smp30 probe. Inhibited proximal tubule formation in prdx1 morphants was rescued by WT prdx1 but not with mutant prdx1* RNAs. (D) Graphical demonstration of smp30 expression in embryos co-injected with WT or mutant prdx1 (M1, M2 or M1/M2) and prdx1 MOs compared with the controls. Image published in: Chae S et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 33 and 34 proximal tubule pronephric kidney Image source: Published Larger Image Printer Friendly View

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