XB-IMG-175730
Xenbase Image ID: 175730
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Figure 4. Prdx1 regulates pronephrogenesis via the Wnt signaling pathway by modulating ROS levels in X. laevis embryos. (A) prdx1 MOs and WT prdx1*, WT Dsh (Dsh WT), DshδDEP, or DshδDIX were co-injected into both blastomeres of two-cell stage embryos. Embryos at the stage 33 were used for whole-mount in situ hybridization, and proximal tubules in the pronephros were visualized with a smp30 probe. Pronephros development recovered only in embryos co-injected with WT prdx1* or Dsh WT. (B) Graphical demonstration of pronephros development in embryos co-injected with prdx1 MOs and WT prdx1*, Dsh WT, DshδDEP, or DshδDIX. (C) MDCK cells were transfected with either 10 nM prdx1 or control siRNAs. Primary cilia were visualized using an acetylated-tubulin (Ac-Tub) antibody. Cell nuclei were stained with DAPI. Numbers of cilia cells were markedly reduced in MDCK cells by prdx1 siRNA mediated knockdown of prdx1. (D) Graphical representation of ciliated cells transfected with control siRNA and prdx1 siRNA. prdx1 knockdown significantly reduced the number of cilia in MDCK cells. (E) Specificity of prdx1 knockdown in MDCK cells transfected with Prdx1siRNA was confirmed by RT-PCR examination as well as western blot studies. Significant reduction in levels of prdx1 mRNA and proteins was observed in MDCK cells transfected with Prdx1siRNA. (F) Control and prdx1-injected (200 pg) embryos were treated with 2 μM H2O2 at stage 8.5. Embryos at the stage 33 were used for whole-mount in situ hybridization, and proximal tubules in the pronephros were visualized with a smp30 probe. H2O2-disrupted proximal tubule formation in the pronephros, and abnormal proximal tubule formation was rescued in Prdx1-injected embryos. (G) Graph showing the pronephros development in embryos co-treated with H2O2 and prdx1. Reduced expression of smp30 in H2O2 treated embryos was rescued by prdx1.
Image published in: Chae S et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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