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Figure S2. Injection of the prdx1 MOs results in phenotypic abnormalities. A. prdx1 MOs were injected into both blastomeres of two-cell stage embryos. There were phenotypic abnormalities in prdx1 MO-injected embryos compared with control MO- injected embryos. B. Severity of the phenotypic abnormalities in prdx1 MO-injected embryos compared with control MO-injected embryos. C. prdx1 MO was injected into a V.2.2 blastomere of 16-cell stage embryos. Embryos at the stage 33 were used for whole-mount in situ hybridization, and proximal tubules in the pronephros were visualized with a smp30 probe. Embryos exhibited similar developmental abnormalities as were observed for prdx1 MO injections at two-cell stage. D. prdx1 MOs (40 ng) were injected into both blastomeres of two-cell stage embryos. Embryos at the stage 33 were transversely sectioned, and serial sections were stained with hematoxylin and eosin. The prdx1 MO-injected embryos displayed malformed or undifferentiated internal organs that appeared to be scattered. Nt, neural tube; Sm, somites; Pn, pronephros; Da, dorsal aorta. E. Specificity of prdx1 MO was confirmed by western blot analysis using Flag antibody. The translation product for WT prdx1 RNA was markedly reduced by prdx1 MO. F. 4A6 staining of intermediate, distal and connecting tubules was performed at stage 40 in prdx1 morphants. prdx1 knockdown did not affected formation of intermediate, distal and connecting tubules. Image published in: Chae S et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 33 and 34 proximal tubule pronephric kidney Image source: Published Larger Image Printer Friendly View

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