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Figure S3. Transfection with Prdx1 siRNAs increased the ROS levels in MDCK cells. A. Endogenous ROS levels in MDCK cells transfected with either 10 nM prdx1 or control siRNAs were measured using flow cytometry. The cellular ROS level was higher in prdx1 siRNA-transfected cells than in control siRNA-transfected cells. B. MDCK cells were co-treated with H2O2 and H2O2 + prdx1 and observed their effects on ROS products. Treatment of H2O2 enhanced the phosphorylation of AKT (P-AKT) while phosphorylation of AMPKα (P-AMPKα) was reduced. Prdx1 transfection reversed the expression i.e. downregulated the expression of P-AKT and upregulated the P-AMPKα expression. Induced P-ERK by H2O2 was not affected by expression of prdx1 in transfected MDCK cells. Image published in: Chae S et al. (2017) © The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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