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Figure 2. Cdc42ep3-MO affected the expression of somitic genes. (AC) Embyros were injected with 10 ng of CEP3-MO on one side, and the expression of somitic genes were compared between injected and uninjected sides. At the late gastrula stage (stage 15), CEP3-MO did not affect the expression of mesodermal genes myf5 or myod1 or pan-mesodermal gene tbxt (Xbra). At the early neurula stage (stage 22), the expression of mef2d and myod1 shows a segmented pattern in the uninjected side, but not in the CEP3-MO-injected side (arrows). At late tailbud stages, the segmented expression pattern, but not the overall expression level of myogenic genes myog and myf6 was affected by CEP3-MO. Similar segmentation defect was observed in embryos stained with 12/101 antibody. (D) While 0.3ng CEP3 alone did not induce obvious segmentation defect, coinjection of CEP3 with CEP3-MO partially rescued the segmentation defects. Dorsal view (A, top panels in B, and top panels in D) or lateral view (bottom panels in B,C, and bottom panels in D, showing both sides of the same embryo) of the embryos was shown, with anterior to the left. (E) The percentage of defective embryos were summarized in the bar graph. Chi-squared test was performed and both CEP3-MO mediated knockdown and CEP3 rescue led to significant difference in the phenotype. *P < 0.01. Image published in: Kho M et al. (2019) Copyright © 2019 Kho, Shi and Nie. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue myf5.L myf-5, Xmyf-5, Xmyf5 X. laevis Throughout NF stage 15 paraxial mesoderm myod1.L mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 paraxial mesoderm tbxt.L bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 15 mesoderm notochord circumblastoporal collar mef2d.L mef2d-a, mef2d-b, sl1, XMEF2D X. laevis Throughout NF stage 22 somite presomitic mesoderm myod1.L mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 22 somite presomitic mesoderm myog.L myf 4, myf-4, myf4, myog-a, myog-b, myogenin, Xmyog, XMyogenin X. laevis Throughout NF stage 28 somite myf6.L herculin, mrf4, myf6-a, myf6-b, Xmrf4, XMRF4-a, XMRF4a X. laevis Throughout NF stage 28 somite Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + cdc42ep3 MO + NF22 (in situ hybridization) Fig. 2 BE Anatomical Phenotype abnormal presomitic mesoderm abnormal sclerotome abnormal somite morphology Xla Wt + cdc42ep3 MO + NF28 (in situ hybridization) Fig. 2 CE Anatomical Phenotype abnormal presomitic mesoderm abnormal sclerotome abnormal somite morphology Xla Wt + cdc42ep3 MO + NF35/36 (immunohistochemistry) Fig. 2 CE Anatomical Phenotype abnormal presomitic mesoderm abnormal sclerotome abnormal somite morphology Larger Image Printer Friendly View

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