Xenbase Image ID: 175917

Figure 3. Loss of Cdc42ep3 disrupted somite segmentation and cell shape changes. Wilson explants were dissected from embryos receiving membrane-tethered EGFP (EGFP-CAAX) on one side, and CEP3-MO plus EGFP-CAAX on the other side, and the segmentation process was followed by time-lapse microscopy. (AF) Time frames at 2-hr intervals. The dashed lines in (A) marks the boundaries between pre-somitic mesoderm and the notochord. Arrowheads marked segmentation furrows. Two cells on each side of the explant were followed by magenta shade. CEP3-MO not only impaired somite segmentation, but also affected the mediolateral elongation of cells. Scale bar = 100 um. Image published in: Kho M et al. (2019) Copyright © 2019 Kho, Shi and Nie. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Experiment + Assay Source Phenotypes and Disease Xla wt + cdc42ep3 MO + eGFP + explant + NF17-22 (Other Detection Assay) Anatomical Phenotype abnormal presomitic mesoderm abnormal somite morphology Larger Image Printer Friendly View

Return to previous page