Xenbase Image ID: 175918

Figure 4. Cdc42 needs to be tightly controlled during somite segmentation. (A) 0.05 ng of constitutively active (CA) or dominant negative (DN) Cdc42 was injected into one side of the embryo, and their effect on somite segmentation was examined by the expression of myod1. Comparing to the uninjected control side, the segmented expression pattern of myod1 was lost on the injected side (arrows), suggesting that both increased and decreased activity of Cdc42 will affect somite segmentation. (B) CA-Cdc42 was co-injected with CEP3-MO, and CEP3 or CEP3-MO was co-injected with DN-Cdc42 to determine whether they can rescue segmentation defects caused by CEP3 or Cdc42 knockdown. The percentage of embryos with segmentation defect was summarized in the bar graph. chi-2 test indicates that CA-Cdc42 failed to rescue segmentation defects caused by CEP3-MO (p = 0.52), but CEP3 significantly rescued segmentation defects caused by DN-Cdc42 (*p < 0.01). Coinjection of CEP3-MO with DN-Cdc42, on the other hand, further inhibited somite segmentation (p = 0.02 when comparing with DN-Cdc42 alone). Image published in: Kho M et al. (2019) Copyright © 2019 Kho, Shi and Nie. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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