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Figure 5. CEP3-MO regulates Cdc42 activity in the developing somite. GFP-wGBD that binds to active Cdc42 was expressed in the paraxial mesoderm alone or together with CEP3-MO. (A) While GFP-wGBD was largely internalized and distributed in a diffused manner in control somitic cells, it was localized to the cell membrane in CEP3-MO-injected cells, reflecting a higher level of Cdc42 activity. (B) An intensity plot of GFP-wGBD across the mediolateral axis of cells was generated. There was a significantly higher level of GFP-wGBD at the cell periphery in CEP3-MO-injected cells and a significantly lower level of GFP-wGBD in the center of CEP3-MO-injected cells. Error bars: standard error of the mean. Student t-test was performed, and the gray area marks places where p > 0.01. Image published in: Kho M et al. (2019) Copyright © 2019 Kho, Shi and Nie. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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