Xenbase Image ID: 175976

Figure 3. Vegt and Otx1 Bind to the Chromatin near Mesendodermal Genes Pre-ZGA (A) Venn diagram of Otx1 and Vegt biologically reproducible peak overlaps. (B) Genome browser view of Otx1 and Vegt ChIP-seq near endodermal (nodal6, sox17b.1, sox17b.2, mixer, mix1), mesodermal (fgf20), and mesendodermal (gsc, hhex) genes. (C) Genomic annotation of the location of all Vegt, all Otx1, and Vegt+Otx1 overlapping peaks. (D) Gastrula stage expression pattern of genes associated with all Vegt, all Otx1, and Vegt+Otx1 overlapping peaks. Genes were assigned as “endoderm,” “mesoderm,” or “ectoderm,” based on which germ layer showed the highest expression in TPM. (E and F) Chromatin binding of Vegt (E) and Otx1 (F) near genes involved in germ layer formation identified using ChIP-qPCR on 32-cell stage embryos. eef1a1 and ins promoters were used as negative control. The error bars represent the variation from 3 technical replicates. Image published in: Paraiso KD et al. (2019) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

Return to previous page