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Figure S1. Screen for core maternal endodermal transcription factors. Related to Figure 1. (A) Validation of 8-cell stage dissections by RT-qPCR of animally- and vegetally-localized RNAs. dicer1 was used for normalization. Error bars represent variation from three technical replicates. (B-D) RNA-seq expression in transcripts per million (TPM) of known localized RNAs. Experiments were performed in biological triplicates. vegt (B) and gdf1 (C) RNAs are vegetally-localized, while foxi2 (D) is animally-localized. (E) Average expression of genes in animal and vegetal blastomeres in log2-transformed TPM. Differentially expressed genes are highlighted in green (vegetal) or orange (animal). (F) GO analysis of maternally-expressed localized genes.

Image published in: Paraiso KD et al. (2019)

Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license

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