Xenbase Image ID: 175998

Figure 2. Nascent Zygotic Transcription during Early Embryogenesis. (A) Confocal images of EU-RNA (red) and DNA (green) in the animal pole of embryos at ∼C13 with or without EU or indicated amount of α-amanitin. Scale bars, 20 μm. (B) Box and whiskers plot of EU-RNA intensity in individual nuclei at the animal pole of embryos (n > 60 cells for each group). Statistical difference determined by one-way ANOVA. ∗∗∗∗p < 0.0001; n.s., not significant. (C) qRT-PCR quantification for indicated genes with or without EU or α-amanitin. Data are represented as mean ± standard deviation. Statistical difference determined by one-way ANOVA. ∗∗∗∗p < 0.0001. (D) RNA-seq for nascent EU-RNA and total RNA isolated from EU-injected and normal embryos, respectively, at ∼9 h pf (mid-ZGA). Table shows number of genes with DESeq2 normalized reads > 10. (E) Levels of highly induced zygotic genes from RNA-seq for nascent EU-RNA and total RNA. The inset schematic shows selection of highly induced zygotic genes. The inset plot shows correlation of highly induced genes between nascent EU-RNA and total RNA. Slope indicates ∼4-fold higher read-depth in nascent EU-RNA-seq. Linear fitting. (F) Levels of all genes ranked by log2-fold change. The genes more sensitive to detection in EU are highlighted in yellow, and the exclusively maternal genes are shown in light blue. Table shows number of genes with DESeq2 normalized reads > 10. Image published in: Chen H et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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