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Figure S4. Patterning of Large-scale ZGA is Explained by Cell Size, and Elevated Levels of Histones Correlates to A More Stringent Cell Size Threshold. Related to Figure 4. (D, E, F). (D and E) EU-RNA accumulation within individual nucleus as a function of cell diameter for animal pole in embryos containing log2 cell no. = 13.2 and vegetal pole in embryos containing log2 cell no. = 13.2 and 13.8. Data binned in 1 µm increments of cell diameter and represented as mean ± 95% CI. Data fit using Hill function, with 95% CI band. Dotted lines indicate 10% of cells being activated. (F) Cell size threshold for transcription at the animal and vegetal pole. We define ZGA initiation for a bin of cells as 10% of cells being activated. Here this occurs at a size threshold of ~ 45 µm at the animal pole and ~ 62 µm at the vegetal pole. Data fit using Hill function, with 95% CI band. Image published in: Chen H et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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