Xenbase Image ID: 176157

Figure 5. Clk2 cooperates with BMP inhibition to induce neural tissue. (a) Neural‐inducing activity of Clk2 was enhanced in the presence of δBMPR. clk2‐GR (250 pg and 500 pg), clk2 SYA‐GR (250 pg and 500 pg) and δBMPR (270 pg) mRNAs were injected into the animal region of X. laevis embryos at the 4‐cell stage. At the blastula stage, embryos were transferred to a medium containing DEX and the ectodermal explants were isolated. “Embryo” and “−RT” indicate whole embryos examined in the presence or absence of reverse transcriptase, respectively. histone h4 was used as the loading control. (b–g) Together with δBMPR, Clk2 induced the formation of a partial secondary axis (arrowheads) at neurula stages. (h) Percentage of partial secondary axis induced by Clk2 and δBMPR. Image published in: Virgirinia RP et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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