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Figure 6. Clk2 promotes the development of posterior neural tissues in cooperation with FGF signaling. (a) Clk2 effectively induced neural tissue in combination with FGF signaling. clk2‐GR (250 and 500 pg), clk2 SYA‐GR (250 pg and 500 pg) and efgf (1 pg) mRNAs were injected into the animal region of X. laevis embryos at the 4‐cell stage. At the blastula stage, embryos were transferred to a medium containing DEX and the ectodermal explants were isolated. “Embryo” and “−RT” indicate whole embryos examined in the presence or absence of reverse transcriptase, respectively. histone h4 was used as the loading control. (b–g’) Coinjection of clk2‐GR or clk2 SYA‐GR together with efgf mRNA induced the formation of a tail‐like structure (arrowheads). (h) Percentage of tail‐like structure induced by Clk2 and eFGF. c’, e’ and g’: magnification of c, e and g, respectively Image published in: Virgirinia RP et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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