XB-IMG-176159
Xenbase Image ID: 176159
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Figure 7. Clk2 modulates BMP and FGF signals. (a) Clk2 decreases the level of pâSmad1/5/8 in cooperation with BMP signal inhibition. clk2 SYAâGR (250 pg and 500 pg) and δBMPR (135 pg) mRNAs were injected into the animal region of X. laevis embryos at the 4âcell stage. At the blastula stage, embryos were transferred to a medium containing DEX and the ectodermal explants were isolated. At the midâgastrula stage (stage 11.5), whole cell extracts were immunoblotted with antiâphospho Smad1/5/8 (pâSmad1/5/8), antiâSmad1 (Smad1), and antiâtubulin antibodies. Tubulin was used as the loading control. (b) Clk2 activates MAPK together with FGF. clk2 SYAâGR (250 pg and 500 pg) and efgf (10 pg) mRNAs were injected into the animal region of X. laevis embryos at the 4âcell stage. At the blastula stage, embryos were transferred to a medium containing DEX and the ectodermal explants were isolated. At the early gastrula stage (stage 10), whole cell extracts were immunoblotted with antiâdiphosphorylated MAPK (dpâMAPK), antiâMAPK (MAPK), and antiâtubulin antibodies. Tubulin was used as the loading control. Image published in: Virgirinia RP et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |