XB-IMG-176375
Xenbase Image ID: 176375
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Figure 1. The snai2:eGFP transgenic embryos show eGFP expression in the CNC lineage at various stages. Heterozygous snai2:eGFP embryos were imaged at the indicated stages. (A–C) Dorsal view of neurula-stage embryos showing eGFP expression in pre-migratory (A), early migrating (B) and extensively migrating (C) CNC, with anterior at the top. Green fluorescence and bright-field images are merged in (B) to show the relative positions of migrating CNC streams in the whole embryo. (D,D’) Side (D) and dorsal (D’) views (with anterior to the left) of the head of a stage ~35 tadpole, with eGFP expression in the developing brain (br), lens (ln), and CNC cells forming condensing mesenchyme in the pharyngeal arches (pa). (E-E”) Dorsal (E), ventral (E’) and side (E”) views (with anterior to the left) of a stage ~42 tadpole. eGFP expression is detectable in the developing olfactory epithelium (oe), trigeminal nerves (nV), and CNC cells in the pharyngeal arches that begin to differentiate into branchial cartilage (bc) and ceratohyal cartilage (cc). (F-F”) Dorsal (F), ventral (F’) and side (F”) views (with anterior to the left) of a stage ~46 tadpole. eGFP is seen in the more differentiated trigeminal nerves and head cartilage structures, including Meckel’s cartilage (mc). Inset in (F”) is a higher-magnification image showing eGFP expression in tail somites. (G,G’) Dorsal (G) and ventral (G’) views (with anterior at the top) of a stage ~48 tadpole, with eGFP visible in both the olfactory (nI) and oculomotor (nIII) nerves. White arrowhead in (G’) indicates strong autofluorescence in the liver, which was also seen in wild-type tadpoles. (H,H’) Dorsal (H) and ventral (H’) views (with anterior at the top) of a stage ~53 tadpole. eGFP labels the thymus (tm) and highly differentiated head cartilage structures. Red scale bar in H = 500 μm. Image published in: Li J et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |