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Figure 3. Localization of eGFP protein in snai2:eGFP embryos. (A–C”) Co-localization of eGFP with endogenous Snai2 protein in snai2:eGFP embryos. Immunohistochemistry was carried out for eGFP (green) and Snai2 (red) simultaneously at the indicated stages in snai2:eGFP embryos and tadpoles. (A-A”) eGFP and Snai2 are co-localized in the CNC at the onset of migration. A control embryo processed with secondary antibodies only but not either primary antibody did not display any signal (insets in A and A’). Embryos are shown in dorsal view with anterior at the top. (B–C”) Dorsal (B-B”) and ventral (C-C”) views of the head of a stage ~46 tadpole showing co-localization of eGFP and Snai2 in the branchial cartilage (bc), brain (br), lens (ln), trigeminal nerve (nV), and olfactory epithelium (oe), with anterior at the top. (D–E”) Transverse sections of anterior head cartilage. Immunohistochemistry for eGFP (green) and DAPI labeling for nuclei (blue) were carried out for stage ~46 snai2:eGFP (D-D”) and wild-type (E-E”) tadpoles, and images were taken with a Zeiss Axiozoom.V16 epifluorescence microscope. Sections are shown with anterior at the bottom (tilted toward the right in D-D”). Expression of eGFP is detectable in Meckel’s (mk) and infrarostral (ir) cartilage in snai2:eGFP but not wild-type tadpoles. Scale bar = 100 μm. Image published in: Li J et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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