Xenbase Image ID: 176381

Figure 7. Inhibition of Wnt signaling in the post-migratory CNC reduces snai2 and sox9 expression. A,B. Wild-type embryos were treated with XAV939 (5 μM), IWR1-endo (10 μM) or DMSO from stage ~28 to ~35, and processed for in situ hybridization for snai2 (A) or sox9 (B). (C,D) One blastomere of 2-cell stage embryos was injected with 50 pg of mRNA encoding EnR-LefΔN-GR755A. Embryos were treated with DEX or DMSO (as control) from stage ~28 to ~35, and processed for in situ hybridization for snai2 (C) or sox9 (D). A representative tadpole from each treatment group is shown in side view in the upper panels (in C,D both the uninjected and injected sides of the same embryos are displayed side by side for comparison), and statistics is shown in the graphs. White arrowheads point to reduced staining in the condensing mesenchyme in the pharyngeal arches. **P < 0.01; ***P < 0.001. Image published in: Li J et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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