Xenbase Image ID: 176451

Fig. 2. Depletion of Rpsa using either a translation (RpsaATG) or splice (RpsaSplice) blocking morpholino impairs expression of nkx2-5 and bapx1 in the spleen of stage 38 rpsa morphants. (A) Representative images of the expression phenotype. Middle and right panels are magnifications of the spleen region marked by a box in the left panel. Black arrowheads indicate normal expression. Blue arrowheads indicate reduced expression. Red arrowheads indicate lack of expression. (B) The percentage of embryos with reduced or absent nkx2-5 or bapx1 expression in the developing spleen following rpsa depletion. ***P≤0.0001 (two-tailed Fisher's exact test). Image published in: Griffin JN et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-5 AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. tropicalis Throughout NF stage 37 and 38 splanchnic layer of lateral plate mesoderm heart nkx3-2 bagpipe homeobox homolog 1, bapx1, nkx3.2, Xbap X. tropicalis Throughout NF stage 37 and 38 splanchnic layer of lateral plate mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + Rpsa MO + NF37/38 (in situ hybridization) Fig. 2 A Anatomical Phenotype abnormal development of spleen Expression Phenotype decreased amount nkx2-5 expression in spleen absent nkx2-5 expression in spleen Xtr Wt + Rpsa MO + NF37/38 (in situ hybridization) Fig. 2 A col 3 Anatomical Phenotype abnormal development of spleen Expression Phenotype absent nkx3-2 expression in spleen decreased amount nkx3-2 expression in spleen Larger Image Printer Friendly View

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