XB-IMG-176587
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FIGURE S4 | Craniofacial defects caused by WHS-associated gene KD are rescued by co-injection of exogenous mRNA co-expression. Facial widths from control, depletion, or rescue strategies were measured in tadpoles (st. 40). Row 1: Embryos injected with (A) control MO (n = 17), (B) 10 ng whsc1 MO (n = 14), or (C) 10 ng of whsc1 MO and 250 pg of whsc1 exogenous mRNA. (D) Comparisons of facial width showed an 8.76% increase in facial width with Whsc1 KD, which was rescued by whsc1 mRNA co-injection. Row 2: Embryos injected with (E) control MO (n = 21), (F) 10 ng whsc2 MO (n = 17), or (G) both 10ng of whsc2 MO and 250 pg of whsc2 mRNA (n = 19). (H) Whsc2 knockdown caused an 8.37% reduction in facial width, which was rescued by exogenous whsc2 mRNA co-injection. Row 3: Embryos injected with (I) control MO (n = 10), (J) 20 ng of letm1 MO, or (K) 20 ng letm1 MO and 1500 pg of letm1 mRNA (n = 11). (L) KD of Letm1 caused a 14.95% decrease in facial width, and was rescued by co-injection of exogenous letm1 mRNA. Row 4: Embryos injected with (M) control MO (n = 9), (N) 20 ng of tacc3 MO (n = 18), or O) 20 ng of tacc3 morpholino and 1000 pg of tacc3 mRNA (n = 16). (P) Tacc3 KD resulted in a 9.01% decrease in facial width, and was rescued by tacc3 mRNA co-injection. Significance determined using a student’s unpaired t-test. ∗∗P < 0.01, ∗P < 0.05, n.s., not significant. Scalebar is 250μm. Image published in: Mills A et al. (2019) Copyright © 2019 Mills, Bearce, Cella, Kim, Selig, Lee and Lowery. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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