Xenbase Image ID: 176889

Fig 4. Validation of cell-cycle enrichment in X. laevis using targeted MS. (A) Workflow of target protein and peptide selection for PRM MS–based validation. (B) Mean protein abundance with SEM in each species by PRM assay is shown. The data were grouped by functional classes for visualization. For all proteins in (B), Xenopus laevis was significantly (p ≤ 0.05) higher than at least one other species. Shown to the right of each graph are examples of the most enriched proteins in each functional class (**p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001). See S16 Table for numerical data underlying figure. DDA, data dependent analysis; MS, mass spectrometry; PRM, parallel reaction monitoring. Image published in: Federspiel JD et al. (2019) © 2019 Federspiel et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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