Xenbase Image ID: 177752

S1. a) Both dorsal blastomeres of Xenopus 4-cell stage embryos were injected with either 4 pmol of the control morpholino (control) or the xLef1 specific morpholino (LefMO). DMZ cells were explanted at the onset of gastrulation and mRNA was isolated when siblings reached late gastrulation (stage 12). Purified mRNA probes (RIN>8) where analyzed on an ATLAS Microarray. The TOP-100 of the differentially regulated genes include the caveolar core components caveolin1 (cav1), caveolin3 (cav3), and cavin1. The total list of the TOP-100 target genes is found in Supplementary Table 1. b) Expression of the caveolar core components during early Xenopus development revealed that cavin1 and caveolin1 (cav1) are co-expressed in the notochord from early tailbud stage on. Caveolin3 (cav3), instead, is expressed in a salt and pepper pattern in multiciliated cells of the epidermis. At gastrula stages, no local accumulation of the caveolin3 mRNA was detectable. c) Lef1 (LefMO) were injected into one blastomere of Xenopus two- cell stage embryos. The asterisks mark the injected site. In situ hybridization revealed that 10 out of 29 of the Lef1 morphants embryos displayed markedly reduced expression of caveolin3 in multiciliated cells of the skin. In Lef1 morphant embryos co-injected with XLef1 mRNA (500 pg) only 2 out of 20 embryos showed reduced cav3 expression. Image published in: Puzik K et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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