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S6. Wnt5a induces disappearance of Fzd7/Ror2 dimers at the apical membrane. a) Animal cap explants were analyzed for localization of total Ror2 (Ror2mCherry, red channel), Ror2 homodimers (splitYFP signal, green channel) and Fzd7/Ror2 dimers (splitYFP signal, green channel) in the absence (-Wnt5a) and presence (+Wnt5a) of co-injected Wnt5a and in the presence of 50 microM genistein. Ten optical sections of 1 microm thickness allowed us to visualize localization of the signals in the z- dimension. The white bar in the close-ups of the yz-plane marks the z-coordinate shown in the xy-images. b) Selected xy-planes at three different z-values illustrate the exclusively basal and baso-lateral localization of Fzd7/ Ror2 dimers in Wnt5a co-injected explants. c) Quantification of the z-distribution of the fluorescence signals, as described in the legend of Figure 5. A total of more than 3,500 splitYFP fluorescence spots and more than 4500 mCherry fluorescence spots were counted in optical planes of 14 explants derived from four independent experiments. Shown is the fraction of fluorescence spots in the xy-plane as a function of the z-coordinate, from basal (0 microm) to apical (9 microm). Obviously, in the presence of Wnt5a, the splitYFP signal (Fzd7/Ror2) is shifted to the basal side. Image published in: Puzik K et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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