Xenbase Image ID: 177802

Figure 5. Membrane depolarisation induced by noggin in the ectoderm requires Trpc1. Control morpholino (CMO) or TRPC1-MO1 was injected into both blastomeres of 2-cell stage embryos. Animal caps were then prepared at late blastula (stage 9) and loaded with the potentiometric dye DiBAC4(3). (A,B) The mean calculated membrane potential revealed membrane depolarisation following the addition of noggin (3 µg/mL, see blue arrow) in animal caps prepared from (A) CMO- or (B) TRPC1-MO1-injected embryos (n = 4 for each). (C,D) The mean calculated membrane potential in isolated ectoderm cells (n = 5 cells) that were dissociated from animal caps prepared from (C) CMO- or (D) TRPC1-MO1-injected embryos. (E) A box plot showing the maximal depolarisation values reached after noggin stimulation of animal caps or dissociated ectoderm cells prepared from CMO-injected embryos (white bars) or TRPC1-MO1-injected embryos (grey bars). The maximal depolarisation values for the animal caps and dissociated cells prepared from CMO- and TRPC1-MO1 embryos were significantly different, (Mann-Whitney test with *P < 0.02 for animal cap recordings, and **P < 0.004 for dissociated cell recordings). Image published in: Néant I et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page