Xenbase Image ID: 177803

Figure 6. trpc1 knock-down impairs the expression of the early neural gene, zic3. Embryos were co-injected at the 8-cell stage into a single dorsal animal blastomere with nuclear β-galactosidase mRNA and: (A) the standard control-MO, (B) a splice-blocking MO (TRPC1 -MO3), (C) TRPC1- MO1, or (D) TRPC1- MO1 plus r-trpc1 mRNA. Embryos were then fixed at stage 14 for subsequent whole-mount in situ hybridization of zic3; see red arrowheads in panel (A). The side of the embryo injected with MO ± r-trpc1-mRNA was confirmed by reaction of β-galactosidase with X-Gal, as shown by the blue labelling on the right side of each embryo; see white arrowhead in panel (A). Scale bar is 500 µm. (E) A bar chart showing the mean ± S.E.M. (n = 17) ratio of the area of zic3 expression on the injected and un-injected sides of the embryo. The asterisks indicate data that are significantly different at p < 0.001 when using one-way ANOVA and the Tukey’s honest significance post-hoc test. Image published in: Néant I et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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