Xenbase Image ID: 177806

Figure S1: Expression of CaV1.x mRNA in X. laevis. (A) RT-qPCR analysis of Cav1.1, Cav1.2, Cav1.3 and Cav1.4 in ectoderm (animal caps) isolated at mid-gastrula (stage 10.5). The expression levels were normalized to the housing keeping gene odc (ornithine decarboxylase). The expression level Cav1.2 mRNA was significantly higher than that of Cav1.1, Cav1.3 and Cav1.4, in animal caps (one way ANOVA with Bonferroni’s test, ****P<0.0001). The data represent the mean ± SEM of 4 independent experiments, with 20 animal caps being used for each experiment. (B) RT-qPCR analysis of Cav1.2 in ectoderm (animal caps) isolated from embryos before gastrulation (stage 8 and stage 9) and at midgastrula (stage 10.5). The expression levels were normalized to the housing keeping gene odc (ornithine decarboxylase). No significant changes in Cav1.2 mRNA levels occurred during these 3 stages (one-way ANOVA with Bonferroni’s test, P<0.05). The data represent the mean ± SEM of 4 and 5 independent experiments for stage 8 and 10.5, and for stage 9, respectively, such that in each experiment there were 20 animal caps. (C-D) Histograms to show RT-qPCR analysis of Cav1.2 (C) and of Cav1.1, Cav1.3 and Cav1.4 (D) in control stage 9 animal caps and in noggin-treated stage 9 animal caps. The level of expression was normalized to the housing keeping gene odc (ornithine decarboxylase). No significant changes in the mRNA levels of Cav1.1, Cav1.2, Cav1.3, and Cav1.4 were observed when comparing control and noggin-treated animal caps (Mann-Whitney test). The data represent the mean ± SEM of 10 independent experiments, with 20 animal caps per experiment. Image published in: Néant I et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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