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Figure S3: Trpc1 knock down abolishes the increase in intracellular Ca2+ generated following the activation of CaV1.2 channels in animal cap explants. (A-B) Relative changes in fluorescence (F/F0) reveal changes in intracellular Ca2+ generated in a single animal cap loaded with the Ca2+ -indicator Fluo4 after being isolated from an embryo injected with either (A) control-MO or (B) TRPC1-MO1. In (A) panels (a-d), and in (B) panels (a-c), the graphs show examples of 4 and 3 independent experiments, respectively. Values are plotted as the F/F0 mean (red traces) + SEM (black bars) from 4 (A) or 10 (B) randomly selected fields within a single animal cap. Noggin (3 µg/mL) was added (blue arrows) within the first 10 min after the start of data acquisition. Image published in: Néant I et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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