XB-IMG-177809
Xenbase Image ID: 177809
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Figure S4: trpc1 knock-down impairs the expression of the early neural gene, sox2. Embryos were
co-injected at the 8-cell stage into a single dorsal animal blastomere with nuclear β-galactosidase
mRNA (150 pg) and either (A,B) the standard control-MO (CMO; 17 ng) or (C-F) TRPC1-MO1 (17 ng).
Embryos were then fixed at stage 14 for subsequent whole-mount in situ hybridization for sox2; see
black arrows. The side of the embryo injected with MO was confirmed by reaction of β-galactosidase
with Red-Gal, as shown by the red labelling on each embryo; see red arrows. (A-B) Images from 2
different embryos injected with CMO showing similar levels of sox2 expression on the left and right
side of the embryos. (C-F) Images from 4 different embryos injected with TRPC1-MO1 showing that
the expression of sox2 was reduced (see black arrows) on the injected side. Scale bar 500 µm Image published in: Néant I et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |