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Figure S6. The truncated form of BmprRII (BmprII-deltaTD) has no neural inducing activity. RT-qPCR analysis of two neural genes, zic3 (A) and sox2 (B), and two genes controlling epidermal fate, msx1 (C) and bmp4 (D), in noggin-treated stage 9 animal caps (grey bars), in animal caps over-expressing bmprII-deltaTD (black bars) and in control animal caps (white bars). Expression was normalized to the housing keeping gene odc (ornithine decarboxylase). When comparing the level of expression of these 4 genes in animal caps over-expressing bmprII-deltaTD, with the level of expression in noggin treated and control animal caps, these data indicate that bmprII-deltaTD has no neural inducing activity, (one way ANOVA with Bonferroni’s test, **P<0.001, ****P<0.0001). The data represent the mean ± SEM of 8 independent experiments, with 20 animal caps in each experiment.

Image published in: Néant I et al. (2019)

© The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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