Xenbase Image ID: 178420

Figure 1. Expression of CaV1.2 mRNA in X. laevis. (A) RT-qPCR analysis of Cav1.1, Cav1.2, Cav1.3 and Cav1.4 in ectoderm (animal caps) isolated before gastrulation at stages 8 and 9. The expression levels were normalized to the housing keeping gene odc (ornithine decarboxylase). The level Cav1.2 mRNA was significantly higher than that of Cav1.1, Cav1.3 and Cav1.4 (one way ANOVA with Bonferroni’s test, ****P < 0.0001). The data represent mean ± SEM of 9 independent experiments such that 20 animal caps were used for each experiment. (B) In situ hybridization to show the pattern of localization of CaV1.2 mRNA in sections acquired at early gastrula (stage 10). (Ba) Photomicrograph of a sagittal section labelled with the anti-sense probe, which shows CaV1.2 expression in the ectoderm (ect) and dorsal mesoderm (mes). There is no CaV1.2 expression in the ventral mesoderm. (Bb) Higher magnification view of the ectoderm (corresponding to the white dashed rectangle in panel (Ba), which shows that CaV1.2 is expressed in the internal layer (int) but not in the external layer (ext). (Bc) Photomicrograph of a sagittal section showing no labelling with the sense probe. In (Ba-Bc), dorsal is to the right and the arrows indicate the dorsal blastopore groove. Scale bars are 300 µm. Image published in: Néant I et al. (2019) © The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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