Xenbase Image ID: 179984

Figure 1STING C-Terminal Modules Control the Balance of Downstream IRF3 and NF-κB Signaling (A) Reconstitution of the cGAS-STING pathway in human cells using a phylogenetically diverse panel of vertebrate STING alleles. Luciferase reporters were used to monitor cGAS-STING dependent interferon β (IFNβ; blue) and NF-κB (orange) responses. Species shown are as follows: 1, human (Homo sapiens); 2, marmoset (Callithrix jacchus); 3, mouse (Mus musculus); 4, cat (Felis catus); 5, seal (Leptonychotes weddellii); 6, cattle (Bos taurus); 7, boar (Sus scrofa); 8, bat (Rousettus aegyptiacus); 9, manatee (Trichechus manatus latirostris); 10, Tasmanian devil (Sarcophilus harrisii); 11, ostrich (Struthio camelus australis); 12, crested ibis (Nipponia nippon); 13, lizard (Anolis carolinensis); 14, turtle (Chelonia mydas); 15, western clawed frog (Xenopus tropicalis); 16, African clawed frog (Xenopus laevis); 17, coelacanth (Latimeria chalumnae); 18, salmon (Salmo salar); 19, zebrafish (Danio rerio); and 20, ghost shark (Callorhinchus milii). (B) Schematics of STING domain organization (TM, transmembrane domain; CDN, cyclic dinucleotide binding domain; CTT, C-terminal tail). Cellular reporter assay as in (A), mapping the motif responsible for enhanced NF-κB signaling to the CTT of zebrafish STING. (C) Cellular reporter assay and schematics as in (B), comparing downstream signaling outputs of human STING with human STING containing the zebrafish STING CTT sequence. The zebrafish STING CTT is sufficient to enhance NF-κB signaling. Cellular reporter assay data are representative of at least three independent experiments. Data are plotted with error bars representing the SD of the mean. Image published in: de Oliveira Mann CC et al. (2019) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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