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Figure 1. 1-cell–stage embryos were injected with either standard control MO or emc1 MO and phenotypically assessed at stage 45. (A) Representative images and measurements of 3 replicates of stage 45 control MO (n = 19) and emc1 MO (n = 21) embryo outflow tract morphology imaged with OCT imaging (dotted yellow line indicates measured diameter). Scale bar: 100 μm. (B) Representative images and percentages of 3 replicates of stage 45 control MO (n = 62) and emc1 MO (n = 55) embryo craniofacial cartilage stained with Alcian blue. Scale bar: 250 μm. (C) Immunoblot of pooled (n = 20) Emc1 protein in control and emc1 knockout/knockdown embryos. (D) Immunoblot of pooled (n = 20) Emc1 protein in emc1 knockdown and EMC1 rescued emc1 knockdown embryos. ****P < 0.0001, ***P < 0.0005 by (A) Student’s t test or (B) Fisher’s exact test. Bars indicate mean and SD. Image published in: Marquez J et al. (2020) Copyright © 2020. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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