Xenbase Image ID: 180269

Figure 4. (A) Immunofluorescence antibody labeling of EMC1 revealed a decrease in its expression after EMC1 siRNA treatment as compared with control siRNA treatments. Multipass membrane proteins (RHODOPSIN, nAChR, FZD2, FZD7) were abnormally localized (n = 10 high power fields per marker per condition done in 3 replicates). Scale bar: 20 μm. (B) Sample traces and measurement of control morphant (n = 30) and emc1 morphant (n = 30) tadpole movement over 10 seconds after stimulation (different colors differentiate distinct tadpoles) over 3 replicates. (C) Labeling of nAChR in the proximal tail of emc1-depleted stage 45 tadpoles showed sparse and less intense expression as compared with control counterparts. Scale bar: 50 μm. (D) Splicing assay for xbp1 in pooled (n = 30 per condition) stage 24 Xenopus embryos displayed increased splicing with emc1 MO depletion compared with control embryos repeated in 4 biological replicates. Tunicamycin treatment acted as positive control. (E) Immunoblotting for Fzd7 showed similar levels of Fzd7 in pooled (n = 30 per stage per condition) emc1 morphants as compared with control morphants at stage 14, but a marked decrease in levels at stage 24. ***P < 0.0005 by Student’s t test. Bars indicate mean and SD. Image published in: Marquez J et al. (2020) Copyright © 2020. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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