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Supplemental Figure 2 F0 CRISPR mosaic knockout of emc1 causes abnormal craniofacial morphology. (A) Representative images and quantitation of Cas9 only (n=48) and Cas9 + emc1 sgRNA injected (n=60) stage 45 embryos stained with Alcian blue over 3 biological replicates. Scale bar indicates 250 μm. (B) Example TIDE analysis of insertion and deletion sizes along with their predicted effects in one embryo. This analysis of single embryos was carried out in 5 replicates to ensure consistent CRISPR mediated targeting. “Other” indicates changes that could not be analyzed via TIDE due to large size of indel. ****p < 0.0001 by Fisher’s exact test. Bars indicate mean and SD. Image published in: Marquez J et al. (2020) Copyright © 2020. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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