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Supplemental Figure 3 Fragment analysis of xbp1 splicing demonstrates increased endoplasmic reticulum stress in emc1 morphants. (A) Examples of traces obtained from fragment analysis for xbp1 spliced and un-spliced forms in 4-5 replicates of 30 pooled stage 24 control embryos, tunicamycin (positive control) treated embryos, and emc1 morphant embryos. (B) Ratios of area under the curve measurements corresponding to peaks of spliced to unspliced forms of xbp1 in stage 24 control embryos, tunicamycin (positive control) treated embryos, and emc1 morphant embryos demonstrate an increase in xbp1 splicing in emc1 morphants. *p<0.05 by Student’s T-test. Bars indicate mean and SD. Image published in: Marquez J et al. (2020) Copyright © 2020. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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