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Supplemental Figure 5 Immunoblot analysis of FZD2 levels reveals proteasomal degradation as the source of FZD2 clearance in EMC1 depleted RPE cells. (A) Immunoblot of FZD2 in cells transfected with a control siRNA and subjected to either cycloheximide treatment alone or cycloheximide and MG132 proteasomal inhibition performed in 3 biological replicates. (B) Immunoblot of FZD2 in cells transfected with EMC1 siRNA and subjected to either cycloheximide treatment alone or cycloheximide and MG132 proteasomal inhibition performed in 3 biological replicates. (C) Normalized densitometry of FZD2 levels from immunoblot assays. Bars indicate mean and SD. Image published in: Marquez J et al. (2020) Copyright © 2020. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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