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Fig. S2: TEM identified CTs in myocardium is c-Kit positive but CD31 negative. Double immunofluorescent staining for anti-c-Kit (red) and CD31 (green) demonstrated c-Kit+ and CD31- cells with very small cell bodies (open arrow), a nucleus (approximately 1:1 ratio of the cytoplasm to the nucleus; white asterisk) and extremely then prolongation (telopode) around trabeculae in the X. tropicalis myocardium (white arrow). Showing that TEM identified CTs express c-Kit, a generally accepted marker of CTs, but not CD31, a unique marker of endothelial cell. A: Anti-c-Kit (red); B: Anti-CD31 (green); C: DAPI; D: merged of A,B and C. Scale bar: 20 micro meters. Tr: Trabecula of the myocardium. Image published in: Lv L et al. (2020) © 2020 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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